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IMAGING GENE EXPRESSION: METHODS AND PROTOCOLS (METHODS IN By Yaron Shav-tal NEW
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eBay item number:186384851247
Item specifics
- Condition
- Brand New: A new, unread, unused book in perfect condition with no missing or damaged pages. See all condition definitionsopens in a new window or tab
- ISBN-10
- 1493963120
- Book Title
- Imaging Gene Expression: Methods and Protocols (Methods in
- Genre
- Radiology
- Item Height
- 10 inches
- ISBN
- 9781493963126
- Subject Area
- Science, Medical
- Publication Name
- Imaging Gene Expression : Methods and Protocols
- Publisher
- Humana Press
- Item Length
- 10 in
- Subject
- Life Sciences / Genetics & Genomics, Radiology, Radiotherapy & Nuclear Medicine
- Publication Year
- 2017
- Series
- Methods in Molecular Biology Ser.
- Type
- Textbook
- Format
- Trade Paperback
- Language
- English
- Item Weight
- 253.7 Oz
- Item Width
- 7 in
- Number of Pages
- Xiii, 368 Pages
About this product
Product Identifiers
Publisher
Humana Press
ISBN-10
1493963120
ISBN-13
9781493963126
eBay Product ID (ePID)
235601116
Product Key Features
Number of Pages
Xiii, 368 Pages
Language
English
Publication Name
Imaging Gene Expression : Methods and Protocols
Publication Year
2017
Subject
Life Sciences / Genetics & Genomics, Radiology, Radiotherapy & Nuclear Medicine
Type
Textbook
Subject Area
Science, Medical
Series
Methods in Molecular Biology Ser.
Format
Trade Paperback
Dimensions
Item Weight
253.7 Oz
Item Length
10 in
Item Width
7 in
Additional Product Features
Dewey Edition
23
Series Volume Number
1042
Number of Volumes
1 vol.
Illustrated
Yes
Dewey Decimal
572.865
Table Of Content
High-Throughput Fluorescence-Based Screen to Identify Factors Involved in Nuclear Receptor Recruitment to Response Elements.- Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center.- Single Molecule Resolution Fluorescent In Situ Hybridization (smFISH) in the Yeast S. cerevisiae.- Measuring Transcription Dynamics in Living Cells Using Fluctuation Analysis Nuclear Poly(A) RNA Movement Within and Among Speckle Nuclear Bodies and the Surrounding Nucleoplasm.- Nuclear Trafficking and Export of Single, Native mRNPs in Chironomus tentans Salivary Gland Cells.- Single mRNP Tracking in Living Mammalian Cells.- Imaging Nascent RNA Dynamics in Dictyostelium.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Single Molecule Tracking.- Single Particle Tracking for Studying the Dynamic Properties of Genomic Regions in Live Cells.- Microscopic Analysis of Chromatin Localization and Dynamics in C. elegans.- Measuring the Dynamics of Chromatin Proteins During Differentiation.- Electron Spectroscopic Tomography of Specific Chromatin Domains.- BAC Manipulations for Making BAC Transgene Arrays.- Spatio-Temporal Visualization of DNA Replication Dynamics.- The Dynamics of DNA Damage Repair and Transcription.- Fluorescence Microscopy-Based High-Throughput Screening for Factors Involved in Gene Silencing.- Actin as a Model for the Study of Nucleocytoplasmic Shuttling and Nuclear Dynamics.- Isochronal Visualization of Transcription and Proteasomal Proteolysis in Cell Culture or in the Model Organism, Caenorhabditis elegan.- Considering Discrete Protein Pools When Measuring the Dynamics of Nuclear Membrane Proteins.- Correlative Microscopy of Individual Cells: Sequential Application of Microscopic Systems with IncreasingResolution to Study the Nuclear Landscape.- Time-Lapse, Photo-Activation, and Photo-Bleaching Imaging of Nucleolar Assembly after Mitosis.- Nucleation of Nuclear Bodies.
Synopsis
As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression. In Imaging Gene Expression: Methods and Protocols , expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms., Part I: Imaging Gene Expression and RNA Dynamics in Cells and Organisms 1. High-Throughput Fluorescence-Based Screen to Identify Factors Involved in Nuclear Receptor Recruitment to Response Elements Tina B. Miranda, Ty C. Voss, and Gordon L. Hager 2. Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center Tim Pollex, Tristan Piolot, and Edith Heard 3. Single Molecule Resolution Fluorescent In Situ Hybridization (smFISH) in the Yeast S. cerevisiae Samir Rahman and Daniel Zenklusen 4. Measuring Transcription Dynamics in Living Cells Using Fluctuation Analysis Matthew L. Ferguson and Daniel R. Larson 5. Tracking Nuclear Poly(A) RNA Movement Within and Among Speckle Nuclear Bodies and the Surrounding Nucleoplasm Joan C. Ritland Politz and Thoru Pederson 6. Nuclear Trafficking and Export of Single, Native mRNPs in Chironomus tentans Salivary Gland Cells Tim P. Kaminski, Jan-Hendrik Spille, Claudio Nietzel, Jan Peter Siebrasse, and Ulrich Kubitscheck 7. Single mRNP Tracking in Living Mammalian Cells Alon Kalo, Pinhas Kafri, and Yaron Shav-Tal 8. Imaging Nascent RNA Dynamics in Dictyostelium Jonathan R. Chubb, Michelle Stevense, Danielle Cannon, Tetsuya Muramoto, and Adam Corrigan Part II: Imaging the Genome and Chromatin Dynamics 9. Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Single Molecule Tracking Davide Mazza, Sourav Ganguly, and James G. McNally 10. Single Particle Tracking for Studying the Dynamic Properties of Genomic Regions in Live Cells Irena Bronshtein Berger, Eldad Kepten, and Yuval Garini 11. Microscopic Analysis of Chromatin Localization and Dynamics in C. elegans Christian Lanctôt and Peter Meister 12. Measuring the Dynamics of Chromatin Proteins During Differentiation Arigela Harikumar and Eran Meshorer 13. Electron Spectroscopic Tomography of Specific Chromatin Domains Liron Even-Faitelson, Eden Fussner, Ren Li, Mike Strauss, and David P. Bazett-Jones 14. BAC Manipulations for Making BAC Transgene Arrays Nimish Khanna, Qian Bian, Matt Plutz, and Andrew S. Belmont Part III: Imaging Nuclear Processes and Sub-Nuclear Structures 15. Spatio-Temporal Visualization of DNA Replication Dynamics Marius Reinhart, Corella S. Casas-Delucchi, and M. Cristina Cardoso 16. The Dynamics of DNA Damage Repair and Transcription Niraj M. Shanbhag and Roger A. Greenberg 17. Fluorescence Microscopy-Based High-Throughput Screening for Factors Involved in Gene Silencing Sebastian Bultmann and Heinrich Leonhardt 18. Actin as a Model for the Study of Nucleocytoplasmic Shuttling and Nuclear Dynamics Kari-Pekka Skarp and Maria K. Vartiainen 19. Isochronal Visualization of Transcription and Proteasomal Proteolysis in Cell Culture or in the Model Organism, Caenorhabditis elegan Anna von Mikecz and Andrea Scharf 20. Considering Discrete Protein Pools When Measuring the Dynamics of Nuclear Membrane Proteins Nikolaj Zuleger, David A. Kelly, and Eric C. Schirmer 21. Correlative Microscopy of Individual Cells: Sequential Application of Microscopic Systems with Increasing Resolution to Study the Nuclear Landscape Barbara Hübner, Thomas Cremer, and Jürgen Neumann 22. Time-Lapse, Photo-Activation, and Photo-Bleac, This book will help researchers working towards imaging in the context of complete organisms. It features readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
LC Classification Number
R895-920
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